<aside> ⚠️ This software is in no way affiliated with Lipofectamine 2000 or Lipofectamine 3000 from Invitrogen, or jetPRIME from Polyplus. It is merely a calculation aid tool and may contain errors. Users are advised to independently verify any results obtained from this software before making decisions based on them.
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Cellular transfection is a fundamental technique in molecular biology for introducing nucleic acids, such as DNA or RNA, into living cells. This method plays a crucial role in many areas of research, including cell biology, biotechnology and gene therapy. By enabling targeted genetic manipulation and the study of cellular mechanisms, cell transfection offers promising opportunities for the understanding of biological processes and the development of new therapies.
Transfection efficiency often depends on several factors, including the type of cells used, the transfection method, and optimal ratios of nucleic acids to transfection agents. Among the most commonly used transfection agents are Lipofectamine 2000, Lipofectamine 3000 from Invitrogen, and jetPRIME from Polyplus-transfection. These reagents are designed to enhance the uptake and delivery of nucleic acids into cells, thereby facilitating the transfection process.
This application aims to simplify the process of calculating the necessary ratios between DNA and transfection agents during cell transfection. By providing a user-friendly interface and advanced calculation tools, it allows researchers and scientists to optimize their transfection experiments, explore different parameters and generate personalized experimental conditions. Please note that this application is intended for calculation assistance purposes only and may contain errors. We recommend that you independently verify the results obtained before making decisions based on them.